mycUb-20aa
(Plasmid #20255)

• Depositing Lab: Nico Dantuma
• Publication: Verhoef et al FASEB J. 2009 Jan . 23(1):123-33.

Backbone

• Vector backbone: pCMV-Myc
• Backbone size w/o insert (bp): 3300
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ubiquitin-20 amino acid extension
• Alt name: Ub-20aa
• Species: H. sapiens (human)
• Insert Size (bp): 4063
• Mutation: Ubiquitin fused to N-terminus of GFP. Glycine 76 mutated to Valine. Stop codon introduced after 20 amino acids in GFP
• Entrez Gene: RBX1 (a.k.a. BA554C12.1, RNF75, ROC1)
• Tag / Fusion Protein:
  • myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry