pICKUP2A
(Plasmid
#202604)
-
Purpose(Empty Backbone) Split backbone for transfer of genetic loci to Bacteroides
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202604 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepExchange-tdk
-
Backbone manufacturerEric Martens
- Backbone size (bp) 4172
-
Modifications to backboneInsertion of URA3, CEN6, ARS4
-
Vector typeBacterial Expression, Yeast Expression, Synthetic Biology
-
Selectable markersNeomycin (select with G418) ; Erythromycin (Bacteroides selection), FUDR (Bacteroides counterselection)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Pir1
-
Copy numberLow Copy
Resource Information
-
A portion of this plasmid was derived from a plasmid made bysfGFP cassette from pYTK001
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pICKUP2A was a gift from Johan Larsbrink (Addgene plasmid # 202604 ; http://n2t.net/addgene:202604 ; RRID:Addgene_202604) -
For your References section:
A system for transferring large genetic loci in Bacteroides enables hemicellulose utilization in Bacteroides thetaiotaomicron and characterization of a locus from an uncultivated strain. Porter NT, Kmezik C, Lee Y-H, Siewers V, Pope PB, Koropatkin N, Martens E, Larsbrink J. Appl Environ Microbiol. 2026 Apr 20:e0017626. doi: 10.1128/aem.00176-26. 10.1128/aem.00176-26 PubMed 42007719