pICKUP2B
(Plasmid
#202605)
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PurposeSplit backbone for transfer of genetic loci to Bacteroides
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202605 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepNTP201
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Backbone manufacturerNathan Porter
- Backbone size w/o insert (bp) 1711
- Total vector size (bp) 3392
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Vector typeYeast Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSplit kanMX cassette/split URA3
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1681
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pICKUP2B was a gift from Johan Larsbrink (Addgene plasmid # 202605 ; http://n2t.net/addgene:202605 ; RRID:Addgene_202605) -
For your References section:
A system for transferring large genetic loci in Bacteroides enables hemicellulose utilization in Bacteroides thetaiotaomicron and characterization of a locus from an uncultivated strain. Porter NT, Kmezik C, Lee Y-H, Siewers V, Pope PB, Koropatkin N, Martens E, Larsbrink J. Appl Environ Microbiol. 2026 Apr 20:e0017626. doi: 10.1128/aem.00176-26. 10.1128/aem.00176-26 PubMed 42007719