Salsa-BC11-P1A11
(Plasmid
#202852)
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PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC11
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202852 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCER206
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP/BC11
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SpeciesSynthetic
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Insert Size (bp)730
- Promoter Tet Response Element (TRE)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsrGI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer Custom primer (GAAGCGCGATCACATGG) (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Salsa-BC11-P1A11 was a gift from Noah Palm (Addgene plasmid # 202852 ; http://n2t.net/addgene:202852 ; RRID:Addgene_202852) -
For your References section:
Highly multiplexed bioactivity screening reveals human and microbiota metabolome-GPCRome interactions. Chen H, Rosen CE, Gonzalez-Hernandez JA, Song D, Potempa J, Ring AM, Palm NW. Cell. 2023 Jul 6;186(14):3095-3110.e19. doi: 10.1016/j.cell.2023.05.024. Epub 2023 Jun 14. 10.1016/j.cell.2023.05.024 PubMed 37321219
