pNZ18-CAH
(Plasmid #203159)

• Purpose: Replicates in E. coli, S. cerevisiae, and A. laidlawii
• Depositing Lab: Bogumil Karas
• Publication: Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53. doi: 10.1021/acssynbio.3c00399. Epub 2023 Dec 19.

Backbone

• Vector backbone: pSH71
• Backbone size w/o insert (bp): 5703
• Total vector size (bp): 7081
• Vector type: Bacterial Expression, Synthetic Biology
• Selectable markers: HIS3 ; Kanamycin/Neomycin (for Acholeplasma laidlawii)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): E. coli MC1061
• Growth instructions: Plasmid should be maintained in a RecA+ E. coli strain
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Yeast centromere, autonomously replicating sequence, histidine auxotrophic marker
• Alt name: CEN6, ARSH4, HIS3
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1378

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2022.09.21.508766 for bioRxiv preprint.