pAG416GAL10-WNV_NS2B-GS-NS3
(Plasmid
#203475)
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PurposeExpresses WNV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 marker
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203475 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAG416GAL10-ccdB-6stop
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameWNV NS2B-GS-NS3
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Alt nameWest Nile Virus NS2B-GS-NS3
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Entrez GenePOLY (a.k.a. WNVNY99_gp1)
- Promoter GAL10
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer tggggctctttacatttcca (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG416GAL10-WNV_NS2B-GS-NS3 was a gift from Alejandro Chavez (Addgene plasmid # 203475 ; http://n2t.net/addgene:203475 ; RRID:Addgene_203475)
