pNKX2-5-T2A-GFP-PGK-Puro
(Plasmid
#203607)
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PurposeDonor plasmid to create 3' knockin of NKX2-5 with T2A-GFP
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Depositing Lab
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Depositing OrganizationUniversity of Manchester
Located in the United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203607 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 203607-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 203607-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepUC57
- Backbone size w/o insert (bp) 3522
- Total vector size (bp) 6301
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Vector typeHuman Targeting
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNKX2-5-T2A-GFP-PGK-Puro-NKX2-5
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SpeciesH. sapiens (human)
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Insert Size (bp)3522
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GenBank ID
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Entrez GeneNKX2-5 (a.k.a. CHNG5, CSX, CSX1, HLHS2, NKX2.5, NKX2E, NKX4-1, VSD3)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GTAAAACGACGGCCAGT
- 3′ sequencing primer GTCATAGCTGTTTCCTG
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 203607-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 203607-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNKX2-5-T2A-GFP-PGK-Puro was a gift from Matthew Birket (Addgene plasmid # 203607 ; http://n2t.net/addgene:203607 ; RRID:Addgene_203607) -
For your References section:
The generation and validation of two NKX2-5 fluorescent reporter human embryonic stem cell lines: UMANe002-A-1 and UMANe002-A-2. Douglas M, O'Loughlin C, Lynch AT, Prialgauskaite R, Adamson AD, Dibb KM, Kimber SJ, Birket MJ. Stem Cell Res. 2023 Dec 3;74:103262. doi: 10.1016/j.scr.2023.103262. 10.1016/j.scr.2023.103262 PubMed 38100908
Map uploaded by the depositor.
