PB-NLS-ZapCV2-ALA
(Plasmid #203665)

• Purpose: Expresses zinc dead sensor ZapCV2-Ala in the nucleus of mammalian cells
• Depositing Lab: Amy Palmer
• Publication: Rakshit et al Cell Rep. 2023 Jun 16;42(6):112656. doi: 10.1016/j.celrep.2023.112656.

Backbone

• Vector backbone: piggyback
• Backbone manufacturer: Liu Laboratory at CU Boulder
• Backbone size w/o insert (bp): 6929
• Total vector size (bp): 8505
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ZapCV2-Ala zinc dead sensor
• Alt name: NLS-ZapCV2-Ala
• Species: Synthetic
• Insert Size (bp): 1576
• Mutation: Cys and His in Zap1 is mutated with Ala
• GenBank ID:
• Promoter: CMV promoter
• Tag / Fusion Protein:
  • SV40 NLS (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BanHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: BGH Reverse

Resource Information

• Supplemental Documents:
  • Rakshit_V6N_2_NLS-Zapcv2-Ala-PB_pLann.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry