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PB-NLS-ZapCV2-ALA
(Plasmid #203665)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203665 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 203665-D50 50 μg of DNA in Tris buffer $495
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DNA 203665-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    piggyback
  • Backbone manufacturer
    Liu Laboratory at CU Boulder
  • Backbone size w/o insert (bp) 6929
  • Total vector size (bp) 8505
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ZapCV2-Ala zinc dead sensor
  • Alt name
    NLS-ZapCV2-Ala
  • Species
    Synthetic
  • Insert Size (bp)
    1576
  • Mutation
    Cys and His in Zap1 is mutated with Ala
  • GenBank ID
  • Promoter CMV promoter
  • Tag / Fusion Protein
    • SV40 NLS (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BanHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 Forward
  • 3′ sequencing primer BGH Reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-NLS-ZapCV2-ALA was a gift from Amy Palmer (Addgene plasmid # 203665 ; http://n2t.net/addgene:203665 ; RRID:Addgene_203665)

  • For your References section:

    Human cells experience a Zn(2+) pulse in early G1. Rakshit A, Holtzen SE, Lo MN, Conway KA, Palmer AE. Cell Rep. 2023 Jun 16;42(6):112656. doi: 10.1016/j.celrep.2023.112656. 10.1016/j.celrep.2023.112656 PubMed 37330912
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