pENTR-nlsABACUS2-400n
(Plasmid
#203726)
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PurposeGateway entry vector for the the nlsABACUS2-400n biosensor for (+)-Abscisic acid
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Depositing Lab
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Depositing OrganizationUniversity of Cambridge
Located in the United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203726 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 203726-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 203726-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepDONR221-f1
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Backbone manufacturerInvitrogen
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Modifications to backbonef1 origin into the NspI site of pDONR221
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenlsABACUS2-400n
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SpeciesA. thaliana (mustard weed), Synthetic
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Insert Size (bp)2394
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Tag
/ Fusion Protein
- Myc (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.10.19.512731v1 for bioRxiv preprint.
DNA (Catalog # 203726-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 203726-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-nlsABACUS2-400n was a gift from Alexander Jones (Addgene plasmid # 203726 ; http://n2t.net/addgene:203726 ; RRID:Addgene_203726) -
For your References section:
Next-generation ABACUS biosensors reveal cellular ABA dynamics driving root growth at low aerial humidity. Rowe J, Grange-Guermente M, Exposito-Rodriguez M, Wimalasekera R, Lenz MO, Shetty KN, Cutler SR, Jones AM. Nat Plants. 2023 Jun 26. doi: 10.1038/s41477-023-01447-4. 10.1038/s41477-023-01447-4 PubMed 37365314
