pHLP712
(Plasmid
#203741)
-
PurposeIntegration of OsTIR1(F74A)-3xMyc at NEUT5L locus
-
Depositing Lab
-
Depositing OrganizationPurdue University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203741 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 203741-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 203741-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backboneunknown
- Total vector size (bp) 9864
-
Vector typeCandida albicans integrating vector
-
Selectable markersURA3
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameOsTIR1
-
SpeciesOryza sativa
-
Insert Size (bp)1725
-
MutationCodon 74 changed from Phe to Ala (F74A)
- Promoter ACT1 (C. albicans)
-
Tag
/ Fusion Protein
- 3xMyc (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer none
- 3′ sequencing primer none
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
OsTIR1 gene was made synthetically and codon-optimized for Candida albicans
DNA (Catalog # 203741-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 203741-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHLP712 was a gift from Mark Hall (Addgene plasmid # 203741 ; http://n2t.net/addgene:203741 ; RRID:Addgene_203741) -
For your References section:
Rapid, efficient auxin-inducible protein degradation in Candida pathogens. Milholland KL, Gregor JB, Hoda S, Piriz-Antunez S, Duenas-Santero E, Vu BG, Patel KP, Moye-Rowley WS, Vazquez de Aldana CR, Correa-Bordes J, Briggs SD, Hall MC. mSphere. 2023 Aug 18:e0028323. doi: 10.1128/msphere.00283-23. 10.1128/msphere.00283-23 PubMed 37594261
Map uploaded by the depositor.
