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Addgene

EGFR-mEGFP
(Plasmid #203774)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203774 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Backbone size w/o insert (bp) 2925
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EGFR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4389
  • Entrez Gene
    EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
  • Tag / Fusion Protein
    • mEGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFR-mEGFP was a gift from Thorsten Wohland (Addgene plasmid # 203774 ; http://n2t.net/addgene:203774 ; RRID:Addgene_203774)

  • For your References section:

    Heptanol-mediated phase separation determines phase preference of molecules in live cell membranes. Gupta A, Lu D, Balasubramanian H, Chi Z, Wohland T. J Lipid Res. 2022 Jun;63(6):100220. doi: 10.1016/j.jlr.2022.100220. Epub 2022 Apr 28. 10.1016/j.jlr.2022.100220 PubMed 35490741
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