AAV-U6-sgRNA-hSyn1-DIO-EYFP-KASH
(Plasmid
#203849)
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PurposeNeuron specific expression of a Cre-dependent EYFP tagged to the nuclear membrane through the KASH domain, with an empty gRNA cassette
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203849 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneAAV
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Vector typeAAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEYFP-KASH
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SpeciesM. musculus (mouse)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-U6-sgRNA-hSyn1-DIO-EYFP-KASH was a gift from Ivo Spiegel (Addgene plasmid # 203849) -
For your References section:
Meso-seq for in-depth transcriptomics in ultra-low amounts of FACS-purified neuronal nuclei. Apelblat D, Roethler O, Bitan L, Keren-Shaul H, Spiegel I. Cell Rep Methods. 2022 Jul 25;2(8):100259. doi: 10.1016/j.crmeth.2022.100259. eCollection 2022 Aug 22. 10.1016/j.crmeth.2022.100259 PubMed 36046622
