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Addgene

Anti-DNA-directed RNA polymerase subunit beta' [PR-mAb NT73]
(Antibody #203884)

Ordering

Item Catalog # Description Quantity Price (USD)
Recombinant Antibody 203884-rAb 100 µg of purified recombinant antibody $250 *
Recombinant Antibody trial size 203884-rAb.T 20 µg of purified recombinant antibody $85 *

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Features

Production & Usage

  • Antigen Plasmid
    PVS10
  • Purification
    Purified from cell culture supernatant using affinity chromatography followed by a buffer exchange with centrifugal columns.
  • Storage Buffer
    PBS + 1 mM sodium azide

Delivery

  • Concentration
    0.9–1.1 mg/mL
  • Storage
    Upon receipt, prepare single use aliquots and store at -20 °C until use. Avoid multiple freeze thaw cycles.
  • Shipment
    Blue ice or room temp

Resource Information

  • Persistent Resource Identifier
    RRID:AB_3073516

Terms and Licenses

Target Antigen

Protein DNA-directed RNA polymerase subunit beta'
Antigen Description Purified core RNA polymerase'
Species Other
Gene rpoC
Alternative Names
  • RNAP subunit beta'
  • RNA polymerase subunit beta'
  • Transcriptase subunit beta'
External References

Applications (1)

Western Blot

1 image
Western Blot image by Addgene
Submitted By: Addgene
Antibody Type
Recombinant
Sample Species
Other
Result
Pass
How to cite this antibody ( Back to top)

These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Anti-DNA-directed RNA polymerase subunit beta' [PR-mAb NT73] - from Melina Fan (Addgene antibody # 203884 ; http://n2t.net/addgene:203884 ; RRID:AB_3073516)
  • For your References section:

    Isolation and characterization of a polyol-responsive monoclonal antibody useful for gentle purification of Escherichia coli RNA polymerase. Thompson NE, Hager DA, Burgess RR. Biochemistry. 1992 Aug 4;31(30):7003-8. doi: 10.1021/bi00145a019. 10.1021/bi00145a019 PubMed 1637835