pAttB-Tub-HA-Wnk
(Plasmid
#203968)
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PurposeFly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 203968 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAttB-Tub
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Vector typeInsect Expression
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Selectable markersWhite+
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameWnk (most similar to RO variant)
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SpeciesD. melanogaster (fly)
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Entrez GeneWnk (a.k.a. Dmel_CG7177, CG7177, DWNK, DmWNK, Dmel\CG7177, WNK, cg7177, dWNK, dWnk, wnk)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Lab plasmid #1188
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAttB-Tub-HA-Wnk was a gift from Andreas Jenny (Addgene plasmid # 203968 ; http://n2t.net/addgene:203968 ; RRID:Addgene_203968)