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Addgene

pET3a-UBE2G2
(Plasmid #204209)

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Item Catalog # Description Quantity Price (USD)
Plasmid 204209 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET3a
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UBE2G2
  • Alt name
    MmUbc7
  • Alt name
    NP_003334
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    500
  • Mutation
    none
  • Entrez Gene
    Ube2g2 (a.k.a. 1110003O05Rik, D10Xrf369, UBC7, Ubc7p)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET3a-UBE2G2 was a gift from Allan Weissman (Addgene plasmid # 204209 ; http://n2t.net/addgene:204209 ; RRID:Addgene_204209)

  • For your References section:

    Allosteric activation of E2-RING finger-mediated ubiquitylation by a structurally defined specific E2-binding region of gp78. Das R, Mariano J, Tsai YC, Kalathur RC, Kostova Z, Li J, Tarasov SG, McFeeters RL, Altieri AS, Ji X, Byrd RA, Weissman AM. Mol Cell. 2009 Jun 26. 34(6):674-85. 10.1016/j.molcel.2009.05.010 PubMed 19560420
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