pET24a MDHNP_ARATH
(Plasmid #204238)

• Purpose: Bacterial expression of Arabidopsis chloroplastic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
• Depositing Lab: Malate dehydrogenase CUREs Community (MCC)
• Publication: Expression of His tagged recombinant malate dehydrogenase (MDH) (unpublished)

Backbone

• Vector backbone: pET24a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5268
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MDHNP_ARATH
• Alt name: Arabidopsis chloroplast MDH
• Alt name: acMDH2
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1197
• Mutation: Does not include the chloroplastic transit sequence and includes a MET for start site
• GenBank ID: NM_125218.4
• Entrez Gene: AT5G58330 (a.k.a. AT5G58330, MCK7.20, MCK7_20, NADP-MDH, NADP-dependent Malate Dehydrogenase)
• Promoter: Lac
• Tag / Fusion Protein:
  • 6X His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: T7 Terminal

Resource Information

• Supplemental Documents:
  • pET24a MDHNP ARATH SnapGene
  • MCC Clone Information Sheet - MDHNP_ARATH

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

UniProt ID: https://www.uniprot.org/uniprotkb/Q8H1E2/.

Plays an essential role in the regulation of catalase activity and the accumulation of a hydrogen peroxide-dependent signal by transmitting the redox state of the chloroplast to other cell compartments.

For more information, please see the MDH CUREs Community website: https://www.mdhcures.org/