pQE60 Q58L wgMDH
(Plasmid #204246)

• Purpose: Bacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutant
• Depositing Lab: Malate dehydrogenase CUREs Community (MCC)
• Publication: Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.1111/j.1742-4658.2004.04475.x.

Backbone

• Vector backbone: pQE60
• Backbone manufacturer: Qiagen
• Backbone size w/o insert (bp): 3391
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MDHG_CITLA
• Alt name: watermelon glyoxysomal malate dehydrogenase
• Species: Citrullus lanatus subsp. Vulgaris
• Insert Size (bp): 987
• Mutation: Q58L
• Promoter: T5
• Tag / Fusion Protein:
  • 6X His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (destroyed during cloning)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: CCCGAAAAGTGCCACCTG
• 3′ sequencing primer: CCAGTAATGACCTCAGAAC

Resource Information

• Supplemental Documents:
  • pQE60 Q58L wgMDH SnapGene
  • MCC Clone Information Sheet - wgMDH Subunit Interface Collection
• A portion of this plasmid was derived from a plasmid made by: Received from Leonard J. Banaszak; Original construct from Christine Gietl

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For more information, please see the MDH CUREs Community website: https://www.mdhcures.org/