pJAT77
(Plasmid #204295)

• Purpose: (Empty Backbone) pJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.
• Depositing Lab: David Stern
• Depositing Organization: HHMI, Janelia Research Campus
  Located in United States of America
• Publication: Stern et al bioRxiv 2023.06.17.545412

Backbone

• Vector backbone: Janelia Atalanta cloning plasmid
• Vector type: CRISPR
• Selectable markers: ccdB, SacB

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: Spectinomycin is in the backbone. Chlor and Amp are in the Gateway cloning arms. The plasmids can be maintained on any single of these antibiotics. Depositor typically grows them in Amp or Spec.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pJAT77{attR3-Amp-ccdB-attR4-MCS-attR5-Chlor-SacB-attR6}

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.06.17.545412v1 for bioRxiv preprint.