pJAT58
(Plasmid #204304)

• Purpose: Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.
• Depositing Lab: David Stern
• Publication: Stern et al bioRxiv

Backbone

• Vector backbone: Janelia Atalanta cloning plasmid
• Vector type: CRISPR
• Selectable markers: ccdB, SacB

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: Spectinomycin is in the backbone. Chlor and Amp are in the Gateway cloning arms. The plasmids can be maintained on any single of these antibiotics. Depositor typically grows them in Amp or Spec.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3XP3-DsRed
• Alt name: pJAT58{dU6-3-attR3-Amp-ccdB-attR4-20XUAS-IVS-Syn21-op1-jGCaMP7s-T2A-tdTomato-p10-B3-3xP3-DsRed-B3-attR5-Chlor-SacB-attR6}
• Species: Synthetic
• Promoter: 3XP3

Cloning Information

• Cloning method: Unknown

Resource Information

• Supplemental Documents:
  • pJAT58 GenBank

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.06.17.545412v1 for bioRxiv preprint.