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pRK5-HA-YFP-Raptor K1097R
(Plasmid #204417)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204417 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 204417-D50 50 μg of DNA in Tris buffer $495
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DNA 204417-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pRK5
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4754
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Regulatory Associated Protein Of MTOR, Complex 1
  • Alt name
    raptor
  • Species
    H. sapiens (human)
  • Mutation
    Lysine 1097 to Arginine (K1097R)
  • GenBank ID
    NM_020761
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • YFP (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Mutagensis of pRK5-HA-YFP-raptor (Addgene #73385)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-HA-YFP-Raptor K1097R was a gift from David Rubinsztein (Addgene plasmid # 204417 ; http://n2t.net/addgene:204417 ; RRID:Addgene_204417)

  • For your References section:

    Leucine Signals to mTORC1 via Its Metabolite Acetyl-Coenzyme A. Son SM, Park SJ, Lee H, Siddiqi F, Lee JE, Menzies FM, Rubinsztein DC. Cell Metab. 2019 Jan 8;29(1):192-201.e7. doi: 10.1016/j.cmet.2018.08.013. Epub 2018 Sep 6. 10.1016/j.cmet.2018.08.013 PubMed 30197302
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