pHIT342
(Plasmid #204514)

• Purpose: Bacterial expression of SYP-1 N-terminal fragment with 6xHis and maltose binding protein tags for use as an antigen
• Depositing Lab: Yuji Kohara
• Publication: Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi: 10.15252/embj.2020105002. Epub 2023 Apr 20.

Backbone

• Vector backbone: pHIT198 (derived from pET-28a)
• Backbone size w/o insert (bp): 6442
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: N-terminal fragment of syp-1 (C. elegans)
• Species: C. elegans (nematode)
• Promoter: T7lac
• Tag / Fusion Protein:
  • 6xHis, MBP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: MBP_sense
• 3′ sequencing primer: T7 terminator, reverse primer

Resource Information

• Supplemental Documents:
  • pHIT342.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry