p3xFLAG-p62 var2
(Plasmid
#204576)
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PurposeExpresses 3xFLAG-p62 var in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 204576 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonep3_FLAG-CMV14
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep62
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SpeciesM. musculus (mouse)
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MutationNone
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Entrez GeneSqstm1 (a.k.a. A170, OSF-6, Osi, STAP, STONE14, p62)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3xFLAG-p62 var2 was a gift from Masaaki Komatsu (Addgene plasmid # 204576 ; http://n2t.net/addgene:204576 ; RRID:Addgene_204576) -
For your References section:
Negative regulation of the Keap1-Nrf2 pathway by a p62/Sqstm1 splicing variant. Kageyama S, Saito T, Obata M, Koide RH, Ichimura Y, Komatsu M. Mol Cell Biol. 2018 Jan 16. pii: MCB.00642-17. doi: 10.1128/MCB.00642-17. 10.1128/MCB.00642-17 PubMed 29339380
