pACS37
(Plasmid
#204589)
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PurposeGST-lamin A (506-646)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 204589 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepgex4t1
- Backbone size w/o insert (bp) 4939
- Total vector size (bp) 5434
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman lamin A (506-646)
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SpeciesH. sapiens (human)
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Insert Size (bp)495
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GenBank ID4000
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Tag
/ Fusion Protein
- GST - lamin A (506-646) (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer pGEX3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pACS37 was a gift from Tom Misteli (Addgene plasmid # 204589 ; http://n2t.net/addgene:204589 ; RRID:Addgene_204589) -
For your References section:
Identification of epigenetic modulators as determinants of nuclear size and shape. Schibler AC, Jevtic P, Pegoraro G, Levy DL, Misteli T. Elife. 2023 May 23;12:e80653. doi: 10.7554/eLife.80653. 10.7554/eLife.80653 PubMed 37219077
