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(Plasmid #204597)


Item Catalog # Description Quantity Price (USD)
Plasmid 204597 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pLENTI6.3/V5 -TOPO
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 7692
  • Total vector size (bp) 8101
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    human histone H3.3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    H3-3A (a.k.a. BRYLIB1, H3-3B, H3.3A, H3F3, H3F3A)
  • Promoter CMV
  • Tag / Fusion Protein
    • human histone H3.3 - V5 (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACS141 was a gift from Tom Misteli (Addgene plasmid # 204597 ; ; RRID:Addgene_204597)
  • For your References section:

    Identification of epigenetic modulators as determinants of nuclear size and shape. Schibler AC, Jevtic P, Pegoraro G, Levy DL, Misteli T. Elife. 2023 May 23;12:e80653. doi: 10.7554/eLife.80653. 10.7554/eLife.80653 PubMed 37219077