pET-15b-aδNb072
(Plasmid
#204627)
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PurposeExpresses the anti-IgD nanobody 072 (aδNb072) in the bacterial periplasm. aδNb072 is an orthosteric inhibitor of human anti-Phl p 7 antibodies of clone 102.1F10.
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Depositing Lab
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 204627 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET-15b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5645
- Total vector size (bp) 6146
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameaδNb072
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Alt nameanti-IgD nanobody 072
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SpeciesSynthetic
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Insert Size (bp)501
- Promoter T7
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Tags
/ Fusion Proteins
- TEV protease cleavage site (C terminal on insert)
- His6 (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-15b-aδNb072 was a gift from James McDonnell (Addgene plasmid # 204627 ; http://n2t.net/addgene:204627 ; RRID:Addgene_204627) -
For your References section:
Expanding the Anti-Phl p 7 Antibody Toolkit: An Anti-Idiotype Nanobody Inhibitor. Vester SK, Davies AM, Beavil RL, Sandhar BS, Beavil AJ, Gould HJ, Sutton BJ, McDonnell JM. Antibodies (Basel). 2023 Nov 16;12(4):75. doi: 10.3390/antib12040075. 10.3390/antib12040075 PubMed 37987253
