pHeACT-mEGFP-NLS
(Plasmid
#205003)
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PurposeThe vector contains 1kbp of the upstream and downstream regions of the actin gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.
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Depositing Lab
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Depositing OrganizationNational Institutes of Natural Sciences, Exploratory Research Center on Life and Living Systems
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205003 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 205003-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 205003-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCAGGS
- Backbone size w/o insert (bp) 2021
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Vector typeTardigrade expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Hypsibius exemplaris
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SpeciesHypsibius exemplaris, Tardigrade
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Insert Size (bp)1000
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 205003-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 205003-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHeACT-mEGFP-NLS was a gift from Kazuharu Arakawa (Addgene plasmid # 205003 ; http://n2t.net/addgene:205003 ; RRID:Addgene_205003) -
For your References section:
In vivo expression vector derived from anhydrobiotic tardigrade genome enables live imaging in Eutardigrada. Tanaka S, Aoki K, Arakawa K. Proc Natl Acad Sci U S A. 2023 Jan 31;120(5):e2216739120. doi: 10.1073/pnas.2216739120. Epub 2023 Jan 24. 10.1073/pnas.2216739120 PubMed 36693101
