pRvCAHS3-mEGFP
(Plasmid
#205008)
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PurposeThe vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene positioned in between.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205008 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCAGGS
- Backbone size w/o insert (bp) 2021
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Vector typeTardigrade expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemEGFP flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
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SpeciesRamazzottius varieornatus, Tardigrade
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Insert Size (bp)1000
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRvCAHS3-mEGFP was a gift from Kazuharu Arakawa (Addgene plasmid # 205008 ; http://n2t.net/addgene:205008 ; RRID:Addgene_205008) -
For your References section:
In vivo expression vector derived from anhydrobiotic tardigrade genome enables live imaging in Eutardigrada. Tanaka S, Aoki K, Arakawa K. Proc Natl Acad Sci U S A. 2023 Jan 31;120(5):e2216739120. doi: 10.1073/pnas.2216739120. Epub 2023 Jan 24. 10.1073/pnas.2216739120 PubMed 36693101
