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In vivo expression vector derived from anhydrobiotic tardigrade genome enables live imaging in Eutardigrada.
Tanaka S, Aoki K, Arakawa K
Proc Natl Acad Sci U S A. 2023 Jan 31;120(5):e2216739120. doi: 10.1073/pnas.2216739120. Epub 2023 Jan 24. PubMed Article

Plasmids from Article

ID Plasmid Purpose
205001pRvACT-mEGFP-NLSThe vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.
205002pRvACT-mCherryThe vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.
205003pHeACT-mEGFP-NLSThe vector contains 1kbp of the upstream and downstream regions of the actin gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.
205004pRvCAHS3-mEGFP-NLSThe vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.
205005pRvSAHS1-mEGFP-NLSThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.
205006pRvCAHS3-CAHS3-mEGFP-NLSThe vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the RvCAHS3-mEGFP gene positioned in between.
205007pRvSAHS1-SAHS1-mEGFP-NLSThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the RvSAHS1-mEGFP gene positioned in between.
205008pRvCAHS3-mEGFPThe vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene positioned in between.
205009pRvSAHS1-mCherry-NLSThe vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.
205010pRvUBC-mEGFP-NLSThe vector contains 1kbp of the upstream and downstream regions of the ubiquitin c gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.
205011pRvTUB-mEGFP-NLSThe vector contains 1kbp of the upstream and downstream regions of the tubulin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.
205012pHeEf1a-mEGFP-NLSThe vector contains 1kbp of the upstream and downstream regions of the ef1a gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.
205013pRvCAHS1-mEGFP-NLSThe vector contains 1kbp of the upstream and downstream regions of the cahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.
205014pHeCAHS-mEGFP-NLSThe vector contains 1kbp of the upstream and downstream regions of the chas gene (BV898_02951, CAHS 86272) from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.

Antibodies from Article