T7RNAP Cyslight
(Plasmid
#205077)
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PurposeT7RNAP protein expression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205077 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBR322
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameT7RNAP Cyslight
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SpeciesBacteriophage T7
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MutationC125S, C347S, C510S, C515S, C530S, C723S
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Entrez GeneT7p07 (a.k.a. T7p07)
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
T7RNAP Cyslight was a gift from Smita Patel (Addgene plasmid # 205077 ; http://n2t.net/addgene:205077 ; RRID:Addgene_205077) -
For your References section:
Correlating Transcription Initiation and Conformational Changes by a Single-Subunit RNA Polymerase with Near Base-Pair Resolution. Koh HR, Roy R, Sorokina M, Tang GQ, Nandakumar D, Patel SS, Ha T. Mol Cell. 2018 May 17;70(4):695-706.e5. doi: 10.1016/j.molcel.2018.04.018. 10.1016/j.molcel.2018.04.018 PubMed 29775583
