pET28-hXRCC1pd-HIS
(Plasmid #206036)

• Purpose: Bacterial expression of PAR-deficient hXRCC1pd with 6xHis tag, under the control of T7 promoter
• Depositing Lab: Primo Schaer
• Publication: Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 10.1038/s41467-023-44209-8.

Backbone

• Vector backbone: pET28c(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5206
• Total vector size (bp): 7141
• Modifications to backbone: modified MCS including C-terminal thrombin cleavage site and 6xHIS tag
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: hXRCC1
• Species: H. sapiens (human)
• Insert Size (bp): 1908
• Mutation: S103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A
• Promoter: T7
• Tag / Fusion Protein:
  • HIS (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PstI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Term

Resource Information

• Supplemental Documents:
  • pET-hXRCCpd-HISaddgene.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry