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PurposePlasmid expressing a H2B-mNG2(11) fusion in mammalian cells for complementation with the mNG2(1-10) fragment.
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Depositing Lab
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Depositing OrganizationConcordia University
Located in Canada -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 206043 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 206043-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 206043-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepYTK089
- Backbone size w/o insert (bp) 1874
- Total vector size (bp) 7451
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Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameH2B gene fused to the mNG2(11) fragment
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Alt nameH2B-mNG2(11)
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SpeciesH. sapiens (human)
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Insert Size (bp)435
- Promoter CMV
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Tag
/ Fusion Protein
- H2B-mNG2(11)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer ACGGTGGGAGGTCTATATAAGCAG
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.06.28.546942 for bioRxiv preprint.
DNA (Catalog # 206043-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 206043-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pH2B-mNG2(11) was a gift from Alisa Piekny (Addgene plasmid # 206043 ; http://n2t.net/addgene:206043 ; RRID:Addgene_206043) -
For your References section:
Endogenous tagging using split mNeonGreen in human iPSCs for live imaging studies. Husser MC, Pham NP, Law C, Araujo FRB, Martin VJJ, Piekny A. Elife. 2024 Apr 23;12:RP92819. doi: 10.7554/eLife.92819. 10.7554/eLife.92819 PubMed 38652106
