EGFP Rab11a WT pcDNA3.1 Zeo (+)
(Plasmid #206079)

• Purpose: Rab11a belongs to the Rab family of small GTPases, Rab11 proteins are particularly involved in trafficking through recycling endosomes
• Depositing Lab: Annette Dolphin
• Publication: Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67. doi: 10.1523/JNEUROSCI.2700-10.2010.

Backbone

• Vector backbone: pcDNA3.1 Zeo(+)
• Backbone manufacturer: Invitrogen Life Technologies
• Backbone size w/o insert (bp): 5015
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RAB11A
• Species: H. sapiens (human)
• Entrez Gene: RAB11A (a.k.a. YL8)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CTGGCTAACTAGAGAACC
• 3′ sequencing primer: GTGTCAGCTCCGACTAG

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Prof. T. Hebert

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry