AAV-RO1.7-RPE65
(Plasmid
#206346)
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PurposeAAV plasmid expressing RPE65 in photoreceptors
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 206346 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV-RO1.7-W3SL
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRpe65
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SpeciesM. musculus (mouse)
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Insert Size (bp)1602
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Mutation450-Met (C57BL6 natural variation)
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Entrez GeneRpe65 (a.k.a. 65kDa, A930029L06Rik, LCA2, Mord1, RP20, rd12)
- Promoter 1.7kb human red opsin
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer gacagggctttccatagcAcATGTCTATCCAAATTGAACA
- 3′ sequencing primer ggttgattatctcgagcggGCTAGGATCTTTTGAACAGTC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-RO1.7-RPE65 was a gift from Connie Cepko (Addgene plasmid # 206346 ; http://n2t.net/addgene:206346 ; RRID:Addgene_206346) -
For your References section:
Chromophore supply modulates cone function and survival in retinitis pigmentosa mouse models. Xue Y, Sun X, Wang SK, Collin GB, Kefalov VJ, Cepko CL. Proc Natl Acad Sci U S A. 2023 Jun 6;120(23):e2217885120. doi: 10.1073/pnas.2217885120. Epub 2023 May 30. 10.1073/pnas.2217885120 PubMed 37252956
Map uploaded by the depositor.
