pJethlpAmScarletCm
(Plasmid
#206818)
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PurposeTemplate for hlpA-mScarlet-I amplification associated with chloramphenicol resistance gene
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Depositing Lab
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Depositing OrganizationUniversite de Lorraine
Located in France -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 206818 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 206818-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 206818-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneClonepJetPCR
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Backbone manufacturerThermo Scientific
- Backbone size w/o insert (bp) 2715
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Vector typeSynthetic Biology
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Selectable markersChloramphenicol
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehlpA
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SpeciesStreptococcus pneumoniae
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Insert Size (bp)2197
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Tag
/ Fusion Protein
- mScarlet (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer M13-R(-46)
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byJan-Willem Veening Département de microbiologie fondamentale (DMF), Université de Lausanne
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
chloramphenicol O-acetyltransferase gene under its own promoter is placed downstream of hlpA-mScarlet-I gene ; Please visit https://doi.org/10.1101/2025.05.10.653270 for bioRxiv preprint.
DNA (Catalog # 206818-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 206818-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJethlpAmScarletCm was a gift from Virginie Libante (Addgene plasmid # 206818 ; http://n2t.net/addgene:206818 ; RRID:Addgene_206818) -
For your References section:
A modular genetic toolbox for precise gene regulation and multi-color imaging in streptococci. Mignolet J, Schmid A, Staub J, van der Meer JR, Veening JW, Libante V. Microlife. 2026 Feb 26;7:uqag006. doi: 10.1093/femsml/uqag006. eCollection 2026. 10.1093/femsml/uqag006 PubMed 41834799
