pAAV INS2x mRFP-3MREs (AAV-beta)
(Plasmid #206928)

• Purpose: Beta cell-specific gene expression from AAV in human pancreatic islets
• Depositing Lab: Markus Grompe
• Publication: Chai et al Hum Gene Ther. 2022 Aug;33(15-16):789-800. doi: 10.1089/hum.2021.219. Epub 2022 May 10.

Backbone

• Vector backbone: pAAV INS2x-3MREs
• Backbone manufacturer: Sunghee Chai, Markus Grompe
• Backbone size w/o insert (bp): 4881
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mRFP
• Species: Synthetic
• Insert Size (bp): 678
• Promoter: INSx2

Cloning Information

• Cloning method: Ligation Independent Cloning

Resource Information

• Supplemental Documents:
  • pAAV INS2x mRFP-3MREs (AAV-beta)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The combination of human insulin promoter (INSx2) and 3MREs (microRNA recognition elements) enables transgene expression in beta, and not in alpha cells, in pancreatic islets of human and mouse.