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Addgene

EGFPC1-huNFATc1EE-WT
(Plasmid #24219)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 24219 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFPC1
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NFATc1
  • Alt name
    NFAT2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2147
  • Entrez Gene
    NFATC1 (a.k.a. NF-ATC, NF-ATc1.2, NFAT2, NFATc)
  • Tags / Fusion Proteins
    • GFP (N terminal on backbone)
    • EE (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl II (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer EGFP C seq. primer (#6478-1)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFPC1-huNFATc1EE-WT was a gift from Jerry Crabtree (Addgene plasmid # 24219 ; http://n2t.net/addgene:24219 ; RRID:Addgene_24219)
  • For your References section:

    Nuclear localization of NF-ATc by a calcineurin-dependent, cyclosporin-sensitive intramolecular interaction. Beals CR, Clipstone NA, Ho SN, Crabtree GR. Genes Dev. 1997 Apr 1. 11(7):824-34. 10.1101/gad.11.7.824 PubMed 9106655