SpIMPDH
(Plasmid #206980)

• Purpose: Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.
• Depositing Lab: Vern Schramm
• Publication: Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-2105. doi: 10.1021/acs.biochem.2c00312. Epub 2022 Sep 15.

Backbone

• Vector backbone: pet28b
• Backbone manufacturer: Genscript
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Express protein in One Shot BL21-AI E. Coli
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SpIMPDH
• Alt name: Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase
• Alt name: EC. 1.1.1.205
• Species: Streptococcus pyogenes
• Entrez Gene: guaB (a.k.a. DQM35_RS08830, NCTC12064_01736)
• Tag / Fusion Protein:
  • His tag (with TEV cleavage site) (N terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Genscript

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry