Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20713||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5512
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameCullin 4A
SpeciesH. sapiens (human)
Insert Size (bp)2982
MutationDeletion of residues 594 to 612 from N-terminus
/ Fusion Protein
- HA2 (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Data not available (not destroyed)
- 3′ cloning site Data not available (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
K644R mutation is present but does not affect function
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-HA2-Cul4A-deltaROC was a gift from Yue Xiong (Addgene plasmid # 20713 ; http://n2t.net/addgene:20713 ; RRID:Addgene_20713)
For your References section:Human immunodeficiency virus type 1 Vpr-binding protein VprBP, a WD40 protein associated with the DDB1-CUL4 E3 ubiquitin ligase, is essential for DNA replication and embryonic development. McCall CM, Miliani de Marval PL, Chastain PD, Jackson SC, He YJ, Kotake Y, Cook JG, Xiong Y. Mol Cell Biol. 2008 Sep . 28(18):5621-33. 10.1128/MCB.00232-08 PubMed 18606781