ECFP HO
(Plasmid
#207413)
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PurposeMammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
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Depositing Lab
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Depositing OrganizationStanford University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 207413 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 207413-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 207413-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCEP4 Mammalian Expression Vector
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Backbone manufacturerThermo Fisher
- Backbone size w/o insert (bp) 10400
- Total vector size (bp) 10822
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Modifications to backbone1) Replacement of pCMV with tight-Tet-ON promoter; 2) Cloning of ECFP or mAIRN sequence downstream of tight-Tet-ON promoter
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Vector typeMammalian Expression, Bacterial Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameECFP
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SpeciesSynthetic; Aequorea victoria
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Insert Size (bp)717
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please Note: Addgene NGS is not able to fully resolve the EBNA1 feature within the plasmid due to the repetitive nature of the sequence. Please refer to the depositor's expected sequence for this region of the plasmid.
DNA (Catalog # 207413-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 207413-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ECFP HO was a gift from Karlene Cimprich (Addgene plasmid # 207413 ; http://n2t.net/addgene:207413 ; RRID:Addgene_207413) -
For your References section:
Transcription-Replication Conflict Orientation Modulates R-Loop Levels and Activates Distinct DNA Damage Responses. Hamperl S, Bocek MJ, Saldivar JC, Swigut T, Cimprich KA. Cell. 2017 Aug 10;170(4):774-786.e19. doi: 10.1016/j.cell.2017.07.043. 10.1016/j.cell.2017.07.043 PubMed 28802045
Map uploaded by the depositor.
