pCDNA3 Flag p38 delta
(Plasmid #20785)

• Depositing Lab: Roger Davis
• Publication: Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pDCNA3 Flag p38 delta
• Alt name: Mapk13
• Alt name: Sapk4
• Species: M. musculus (mouse)
• Insert Size (bp): 1998
• GenBank ID: NP_036080
• Entrez Gene: Mapk13 (a.k.a. SAPK4, Serk4)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: Xba I (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: original wild-type p38 delta insert provided by Ulevitch, R.J. and Han,J, "Characterization of the Structure and function of the fourth member of p38 group mitogen-activated protein kinase pathway",(J.BiolChem.272(6),3296-3301 (1997).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry