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(Plasmid #208047)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208047 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
    Derived from Addgene ID# 52962 (Zhang Lab).
  • Vector type
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Mutation
    Cys>Ala mutations in conserved RING domain
  • Entrez Gene
    RNF4 (a.k.a. RES4-26, SLX5, SNURF)
  • Promoter EFS
  • Tag / Fusion Protein
    • BirAopt (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Human codon-optimized BirA was a gift from Oryzko Lab (PMID 15707685). Lenti-EFS/P2A-blast backbone derived from Addgene ID# 52962 (Zhang Lab).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Control for RNF4 BioE3 experiments; contains mutations in the conserved RING domain. RNF4ca is excisable using EcoR1-Not1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-EFS-BirAopt-GSQ-RNF4ca-P2A-BLAST was a gift from Rosa Barrio & James Sutherland (Addgene plasmid # 208047 ; ; RRID:Addgene_208047)
  • For your References section:

    BioE3 identifies specific substrates of ubiquitin E3 ligases. Barroso-Gomila O, Merino-Cacho L, Muratore V, Perez C, Taibi V, Maspero E, Azkargorta M, Iloro I, Trulsson F, Vertegaal ACO, Mayor U, Elortza F, Polo S, Barrio R, Sutherland JD. Nat Commun. 2023 Nov 23;14(1):7656. doi: 10.1038/s41467-023-43326-8. 10.1038/s41467-023-43326-8 PubMed 37996419