OptoProfilin.S138E
(Plasmid #208287)

• Purpose: Expresses Cry2(1-531)-mCherry-Profilin.S138E
• Depositing Lab: Robert Hughes
• Publication: Mann et al ChemBioChem, 8 March 2024

Backbone

• Vector backbone: mCherry N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4018
• Total vector size (bp): 6790
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cry2 - mCherry - Profilin.S138E
• Alt name: profilin-1
• Species: M. musculus (mouse), A. thaliana (mustard weed)
• Insert Size (bp): 2772
• Mutation: Ser 138 (sometimes numbered 137) mutated to Glu
• Entrez Gene: Cry2 (a.k.a. D130054K12Rik)
• Entrez Gene: PRF1 (a.k.a. AT2G19760, F6F22.21, F6F22_21, PFN1, PROFILIN 1, profilin 1)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: pSV40A Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.10.04.560945 for bioRxiv preprint.