pROSA26-SAT
(Plasmid
#208339)
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PurposeROSA26 targeting vector for the SAT sensor for both ERK1/2 and AKT signaling
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Depositing Lab
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Depositing OrganizationIcahn School of Medicine at Mount Sinai
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208339 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 208339-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 208339-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepROSA26-1
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Vector typeMouse Targeting
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSAT sensor
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 208339-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 208339-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pROSA26-SAT was a gift from Philippe Soriano (Addgene plasmid # 208339 ; http://n2t.net/addgene:208339 ; RRID:Addgene_208339) -
For your References section:
Conditional fluorescent mouse translocation reporters for ERK1/2 and AKT signaling. Dinsmore CJ, Soriano P. Dev Biol. 2023 Sep 1:S0012-1606(23)00146-X. doi: 10.1016/j.ydbio.2023.08.007. 10.1016/j.ydbio.2023.08.007 PubMed 37660778
Map uploaded by the depositor.
