pET28-Flag-RBM24
(Plasmid
#208621)
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Purposefor E. coli expression of Flag tagged human RBM24 (ENST00000379052)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 208621 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a(+)
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Backbone manufacturerMerck-Millipore (Novagen)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namehuman RBM24 (ENST00000379052) coding region
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SpeciesH. sapiens (human)
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GenBank IDENST00000379052
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Entrez GeneRBM24 (a.k.a. RNPC6, dJ259A10.1)
- Promoter T7
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Tags
/ Fusion Proteins
- Flag (N terminal on insert)
- 6xHis (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pETupstream, T7p
- 3′ sequencing primer T7ter (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloned from pcDNA3.1 -Flag-RBM24 (Addgene#208052). Sequence between EcoRV and HindIII in pcDNA3.1 (-) was introduced by this clone method.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28-Flag-RBM24 was a gift from Quanfu Ma (Addgene plasmid # 208621 ; http://n2t.net/addgene:208621 ; RRID:Addgene_208621)