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pCAGMKOSiE
(Plasmid #20865)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 20865 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 20865-D50 50 μg of DNA in Tris buffer $495
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DNA 20865-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCAG-ires-EGFP
  • Backbone size w/o insert (bp) 6397
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5a
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    myelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene
  • Alt name
    c-Myc-F2A-Klf4-T2A-Oct4-E2A-Sox2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    4971
  • GenBank ID
    BC006728 NM_011443 NM_013633 NM_010637
  • Entrez Gene
    Myc (a.k.a. Myc2, Niard, Nird, bHLHe39)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGMKOSiE was a gift from Keisuke Kaji (Addgene plasmid # 20865 ; http://n2t.net/addgene:20865 ; RRID:Addgene_20865)

  • For your References section:

    Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Kaji K, Norrby K, Paca A, Mileikovsky M, Mohseni P, Woltjen K. Nature. 2009 Apr 9;458(7239):771-5. doi: 10.1038/nature07864. Epub 2009 Mar 1. 10.1038/nature07864 PubMed 19252477
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