pSR1048
(Plasmid #208738)

• Purpose: Characterization plasmid for PRelA (sfgfp expressed from PRelA on the S. aureus pC194 replicon)
• Depositing Lab: Lauren Andrews
• Publication: Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021/acssynbio.3c00325.

Backbone

• Vector backbone: pCM29
• Backbone size w/o insert (bp): 6603
• Total vector size (bp): 6572
• Modifications to backbone: Changed promoter expressing sfgfp to PRelA
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PRelA
• Species: Bacillus subtilis
• Insert Size (bp): 49
• Promoter: PRelA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BbsI (destroyed during cloning)
• 3′ cloning site: BbsI (destroyed during cloning)
• 5′ sequencing primer: aaatcgacactgaatttgc

Resource Information

• Supplemental Documents:
  • pSR1048.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Chloramphenicol resistance in S. aureus