pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)
(Plasmid
#208977)
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PurposeEntry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208977 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepegRNA entry vector (MNW320)
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
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Alt nameMNW320
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gRNA/shRNA sequencen/a
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SpeciesSynthetic
- Promoter human U6
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Tag
/ Fusion Protein
- BPNLS (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer os280-CAGGGTTATTGTCTCATGAGCGG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.09.12.557440 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320) was a gift from Benjamin Kleinstiver (Addgene plasmid # 208977 ; http://n2t.net/addgene:208977 ; RRID:Addgene_208977) -
For your References section:
Click editing enables programmable genome writing using DNA polymerases and HUH endonucleases. da Silva JF, Tou CJ, King EM, Eller ML, Ma L, Rufino-Ramos D, Kleinstiver BP. bioRxiv. 2023 Sep 13:2023.09.12.557440. doi: 10.1101/2023.09.12.557440. Preprint. 10.1101/2023.09.12.557440 PubMed 37745481
Map uploaded by the depositor.
