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pEP4 E02S ET2K Notes

Addgene diagnostic digest for 20927

This gel depicts the NEB 1kb ladder and a digestion of the plasmid 20927 with the following enzyme: EcoRI-HF. The expected pattern of bands was observed.

Addgene Diagnostic Digest

This gel depicts the NEB 1kb extended ladder and a digestion of the plasmid pEP4 E02S ET2K with the following enzymes: EcoRV-HF, NotI-HF, and ScaI-HF.

Expected Bands
EcoRV-HF:17.5 kb
EcoRv-HF/ NotI-HF:14.4 kb + 3.1 kb
EcoRV-HF/ ScaI-HF:12.7 kb + 4.8 kb

We see the expected bands.