EC70195
(Plasmid
#209456)
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PurposeCloning protospacers for Cas9 editing
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Depositing Labs
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Depositing OrganizationJohn Innes Centre
Located in the United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 209456 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 209456-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 209456-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneUnknown
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Vector typeCRISPR
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Selectable markersLacZ
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas9 sgRNA
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SpeciesSteptococcus pyogenes
- Promoter TaU3
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GoldeGate level 1 position 1
DNA (Catalog # 209456-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 209456-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EC70195 was a gift from Wendy Harwood & Cristobal Uauy (Addgene plasmid # 209456 ; http://n2t.net/addgene:209456 ; RRID:Addgene_209456) -
For your References section:
An optimised CRISPR Cas9 and Cas12a mutagenesis toolkit for Barley and Wheat. Lawrenson T, Clarke M, Kirby R, Forner M, Steuernagel B, Brown JKM, Harwood W. Plant Methods. 2024 Aug 13;20(1):123. doi: 10.1186/s13007-024-01234-y. 10.1186/s13007-024-01234-y PubMed 39138524
