pCF702
(Plasmid #209650)

• Purpose: Suicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicus
• Depositing Lab: Bastian Molitor
• Publication: Fink et al Appl Environ Microbiol. 2023 Jul 26;89(7):e0057523. doi: 10.1128/aem.00575-23. Epub 2023 Jun 13.

Backbone

• Vector backbone: pMTL80150
• Backbone manufacturer: https://plasmidvectors.com (Nigel Minton lab)
• Backbone size w/o insert (bp): 2328
• Total vector size (bp): 5653

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Downstream homologous region
• Insert Size (bp): 1010

Gene/Insert 2

• Gene/Insert name: thermostable neomycin phosphotransferase gene
• Alt name: NeoR
• Insert Size (bp): 1280

Gene/Insert 3

• Gene/Insert name: Upstream homologous region
• Insert Size (bp): 812

Resource Information

• Supplemental Documents:
  • pCF702.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pMTL80150: Heap JT, Pennington OJ, Cartman ST, Minton NP. 2009. A modular system
for Clostridium shuttle plasmids. J Microbiol Methods 78:79–85. https://
doi.org/10.1016/j.mimet.2009.05.004.

thermostable NeoR: pMU131 (Addgene #66811)